What it measures
- Hepatitis B surface antibody (anti-HBs)
Proof of hepatitis B immunity
After a hepatitis B vaccine course, your body makes antibodies against the hepatitis B surface antigen (anti-HBs). This test measures them to confirm you’re protected. A level of 10 mIU/mL or more is generally taken to show immunity, although some employers ask for higher levels.
Your report shows your antibody level, which you can give to your employer or occupational health team. If your level is low, you may need a booster dose.
This test checks immunity, not infection. To check for a current infection, see the Hepatitis B Antigen test.
Who it’s for
- Healthcare and dental workers needing proof of immunity
- Checking your response to a hepatitis B vaccine course
- Travel or occupational health requirements
How to prepare
- Drink plenty of water beforehand; it makes the sample quicker and easier to take.
- You don’t need to fast.
Hepatitis B Antibody (HBsAb) Blood Test: your questions
When should I test after my hepatitis B vaccine?
Usually 1–4 months after completing the vaccine course, unless your employer specifies otherwise.
Information on this page is general and doesn’t replace advice from your GP, midwife or consultant. If you feel unwell or are worried about symptoms, contact your GP, call NHS 111 or, in an emergency, 999.

